A Labrador PeptideAtlas and DIA spectral assay library - resources for proteomics research in dogs.
Proteomics in dogs just got a major upgrade. Researchers have built a Labrador PeptideAtlas—a huge leap for anyone diving into canine peptide research. If you’re working with dog samples, this is your new reference point.
Sci Data
by Kusebauch U, Sun Z, Midha MK et al.
“A Labrador PeptideAtlas and DIA spectral assay library - resources for proteomics research in dogs. Kusebauch U(1), Sun Z(2), Midha MK(2), Kapil C(2), Maes M(2), Baxter DH(2), Morrone SR(2), Reddy PJ(2), O'Flynn C(3), Watson P(3), Deutsch EW(2), Allaway D(3), Moritz RL(4). Author information: (1)Institute for Systems Biology, 401 Terry Ave N, Seattle, WA, 98109, USA. ukusebauch@systemsbiology.org. (2)Institute for Systems Biology, 401 Terry Ave N, Seattle, WA, 98109, USA. (3)Waltham Petcare Science Institute, Waltham-on-the-Wolds, Leicestershire, LE14 4RT, UK. (4)Institute for Systems Biology, 401 Terry Ave N, Seattle, WA, 98109, USA. rmoritz@systemsbiology.org. The domestic dog, Canis lupus familiaris, plays a vital role as companion or service animal, and the well-being and healthy aging of dogs is gaining importance. Proteins are key actors in cells, tissues and body fluids, and the ability to measure their composition and abundance is crucial to understand biological processes. We report a mass spectrometry-based proteomics approach analyzing canine tissues, plasma and urine obtained from Labrador retrievers to develop two comprehensive resources to advance the study of the dog proteome. Firstly, we developed a Labrador PeptideAtlas covering 49% of the predicted UniProtKB proteome and secondly, a Labrador spectral assay library for targeted applications by data-independent acquisition that enables the identification and quantification of 11,792 proteins (11,564 protein groups) of the dog proteome (56%). We demonstrate the performance of the library with gradients of different length and quantify 10,140 proteins in tissues and 385 proteins in plasma. © 2026. Mars Petcare and Institute for Systems Biology. Conflict of interest statement: Competing interests: C.O.F., P.W. and D.A. are employees of Waltham Petcare Science Institute, a part of Mars Petcare. The funders had no role in the design, analysis or writing of this article.”
Here’s what happened: Scientists from the Institute for Systems Biology and Waltham Petcare Science Institute pulled together tissue, plasma, and urine samples from Labrador retrievers. They used mass spectrometry to map out the peptide and protein landscape. The result? Two powerful new resources:
Labrador PeptideAtlas: Covers 49% of the predicted canine proteome. That’s nearly half of all known dog proteins, cataloged and verified for researchers.
Data-Independent Acquisition (DIA) Spectral Assay Library: Enables identification and quantification of 11,792 proteins (11,564 protein groups) in the dog proteome—about 56% coverage.
The team validated the spectral library with different experimental setups. They were able to quantify over 10,000 proteins in tissues and hundreds more in plasma. This isn’t just a list of sequences—it’s a practical toolkit for targeted proteomics in dogs.
Why does this matter? Dogs are key animal models for aging, disease, and even comparative medicine. Having these resources means faster, more accurate peptide and protein analysis, whether you’re trying to track biomarkers, understand disease progression, or optimize canine health.
Key takeaway: If your research touches on canine biology, this is now the gold standard. You’ll find it easier to design your experiments, interpret your data, and push the field forward.
For a deeper dive into peptide research and how these resources fit in, check the peptide research index. This is the kind of groundwork that will drive better science in both veterinary and translational research.
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